After graduating in Musical Sciences & Art Policy and Management at the Utrecht University, Jennie Duif worked for several years as a booker at the Amsterdam Jazz Agency for, among others, Toots Thielemans. In 2005, Jennie became project manager at the Beaufort House in Austerlitz (NL), where she developed several classical and world music series. Until 2010 she was project leader of the  summer festivals Opera! and Festival Behind the Line. In 2015, she led the festivities for the 25th anniversary of the Beaufort House and the associated fundraising campaigns for the refurbishment of the 150-year-old church where it resides. 

Over the years, Jennie has developed various educational projects, such as a retranslation project around Die Dreigroschenoper for secondary school students, in collaboration with Theaterkoor Dario Fo. Together with music schools and primary schools, she made a music theater project that was included in the Children's Make Music program of the Oranje Fonds and the Cultural Participation Fund (NL).

Between 2015 and 2020 Jennie was program manager for the Jazz Orchestra of the Concertgebouw. There she introduced the club tour, which gave the big band a permanent place in the pop circuit. She has also produced many concerts and tours with the orchestra, including those with top soloists such as Joshua Redman, José James, Madeleine Peyroux, Trijntje Oosterhuis and Dr. Lonnie Smith. 

She is currently a member of Reinekwint Project Management and makes programs for the Beaufort House in the field of cultural diversity. Since the beginning of 2021, Jennie has been the business director of Ensemble Black Pencil.